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Image Search Results
Journal: Investigative Ophthalmology & Visual Science
Article Title: Calcium-Dependent Regulation of Desmosomal Proteins in Human Meibomian Gland Epithelial Cells
doi: 10.1167/iovs.66.6.49
Figure Lengend Snippet: Immunohistochemical detection of DSC in the meibomian glands of C57BL/6 mice. ( A ) Acinus (ac) with basal cells (b), differentiating meibocytes (d), and mature meibocytes (m). The immune response is slightly stronger in the peripheral areas of the acinus than in the central areas. The cell borders between individual meibocytes show a stronger immune response and indicate the presence of DSC in the cell membrane. ( B ) Transition of the disintegration zone of an acinus (ac) into the connecting duct (cd). DSC (red reaction product) can be seen in the acini as well as in the epithelia of the connecting and central excretory ducts. Especially the apical layers of the epithelia in particular show a strong immunoreaction. Parts of the ductal system are filled with degenerated cells and meibum in which DSC can also be detected. ( C ) Detection of DSC in the epidermis (epi) of the eyelid serves as a positive control. ( D ) The negative control shows no immunoreaction. The figure shows representative results of three independent experiments ( n = 3). DSC, desmocollin.
Article Snippet:
Techniques: Immunohistochemical staining, Membrane, Positive Control, Negative Control
Journal: Traffic (Copenhagen, Denmark)
Article Title: ER-to-Golgi Blockade of Nascent Desmosomal Cadherins in SERCA2-inhibited Keratinocytes: Implications for Darier’s Disease
doi: 10.1111/tra.12470
Figure Lengend Snippet: (A) Skin tissues derived from a normal subject (a) and DD patients (b–d) were stained with Dsc3. (B) Skin tissues were dual labeled with Dsc3 (red) and ER calnexin (green) and examined in a confocal microscope. Colocalization of Dsc3 and calnexin (yellow signal) is revealed by merged images. Scale bar = 10 μm.
Article Snippet: Following deparaffinization, tissues sections were steamed with antigen retrieval buffer (BioGenex, Citra Plus, pH6.0) and stained with
Techniques: Derivative Assay, Staining, Labeling, Microscopy